Figure 6. No measureable differences in nuclei density or width.

(a- left) The comparison of the nuclei density between the auditory fear conditioning and control groups did not show a significant difference (t-test, n=8 per group, P=0.68, t₁₄=0.422). (a-right) Further, the respective cumulative distribution of the cell nuclei density measures also did not show a significant difference (K–S test, n=96 per group, P=0.68, D=0.1046). (b-left) Likewise, there was an overall lack of a significant difference between the auditory fear conditioning group and the control group for nuclei diameter (t-test, n=8 per group, P=0.42, t₁₄=0.830). (b-right) In addition, the cumulative distribution of nuclei diameters was not significantly different across groups (K–S test, n=2,042 for auditory fear conditioning, n=1,828 for control, P=0.45, D=0.0269). (c–e) The pictures show representative images of the Hoechst staining for the control group (c), auditory fear conditioning group (d) and a combined overlay with the Thy1-YFP-expressing neurons (e). Data in bar graphs are presented as mean±s.e.m. Data in the line graphs are the average of the noted measure (for example, density or width).