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a) Illustration of the labeled PEG sensor in different concentrations of crowding agent (PEG, 10 kDa). Scale bar: 30 Å. b–e) FRET efficiencies plotted as a function of increased cosolute concentration in DPBS buffer for: b) Ficoll 70 and sucrose, c) polyethylene glycol (10 kDa), d) BSA, and e) Xenopus laevis oocyte lysate. Error bars represent mean ±s.d.
