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. 2015 Jul 18;14:280. doi: 10.1186/s12936-015-0801-5

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© Olshina et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Membrane association of PfCoroninHA is mediated by PI(4,5)P₂. a Western blot of PfCoroninHA. Lanes 1 and 2 supernatant (S) and pellet (P) post hypotonic lysis. Lanes 3 and 4 S and P post Na₂CO₃ treatment. Top panel probed with anti-HA (Coronin). Second panel probed with anti-PfADF1 [63]. Third panel probed with anti-MSP1p83 (non-membrane associated protein). Fourth panel probed with anti-MSP1-19 (membrane associated GPI anchor). b–d SPR sensograms of (b) PfCor-N (c) PfADF1 and (d) PH-PLCdelta binding to immobilized PI(4,5)P₂, shown above panel b. Concentrations of each analyte displayed by curves. Determined Kds = inset in graph.