Fig. 3.

Pathway enrichment analysis of differentially expressed gene lists. a Separate gene clusters were defined based on the Venn diagram of MCF7 IGF1R. The mitogenic gene cluster consists of all DEGs in IGF1 treatment alone and the combinations of IGF1 with glargine and/or X10 treatment. Similarly, a metabolic gene cluster was defined including the insulin-specific DEGs with combinations of the other insulin analogues. An Ingenuity Pathway Analysis (IPA) was performed that revealed an enrichment of the ERK/MAPK and p70S6K signaling pathways in the mitogenic cluster whereas the PI3K and cell cycle control signaling pathways were enriched in the metabolic cluster. b An IPA analysis was performed on the DEGs of individual treatments including all cell lines and the different time points. As expected the metabolic signaling (A t/m D) was upregulated after stimulation with metabolic compounds (insulin, glargine and X10). IGF1 stimulation led to a very significant upregulation of PI3K/AKT, ERK/MAPK, IGF1 and p53 signaling. For insulin signaling these pathways were also enriched but less significantly. DEGs differentially expressed genes; IGF1R insulin-like growth factor 1 receptor