Fig. 6.

Validation of insulin analogue mitogenic classifier genes in primary human mammary cells and in vivo mouse mammary glands. a Primary cells isolated from two different human mammary biopsies were exposed to insulin, glargine, X10 or IGF1 and the gene expression of EGR4, TNFRSF11B and SLC1A2 were determined by RT-qPCR. Values are presented as fold changes relative to vehicle-treated cells. b Wild-type FVB mice (four per condition) were subcutaneously injected with the above mentioned compounds, RNA from the mammary glands was isolated and the gene expression of EGR4, TNFRSF11B and SLC1A2 was measured by RT-qPCR and indicated as fold change relative to gene expression of vehicle-injected mice. IGF1 insulin-like growth factor 1