Table 3.
Specimen Repository for INSPIRE Study
| Time Point and Sample Type | A priori testing planned | |
|---|---|---|
| Baseline: | ||
| Infant urine | Markers of inflammation and lung Injury | |
| Infant nasal filter paper* | Respiratory microbiome, cytokines | |
| Infant stool sample* | Gut microbiome | |
| Maternal plasma* | Vitamin E levels | |
| Respiratory Illness: | ||
| Infant urine | Markers of inflammation and lung injury | |
| Infant nasal wash | PCR identification of RSV or RV; cytokines and biomarkers of immune function; microbiome. RSV positive samples will be sequenced. | |
| One Year: | ||
| Infant blood | RSV serology, DNA banking | |
| Home doorframe wipe | Endotoxin/Lipopolysaccharide | |
| Two Year Sub-Study: | ||
| Child peripheral blood mononuclear cells | Immune response to RSV stimulation | |
| Child nasal wash | Nasal microbiome | |
| Child stool sample | Gut microbiome | |
| Three Year: | ||
| Child blood | Serum allergen-specific IgE measurements; if skin prick testing not possible | |
Bold indicates study time point.
*Collected only in second year of enrollment.