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. Author manuscript; available in PMC: 2016 Jul 16.
Published in final edited form as: Cell. 2015 Jul 2;162(2):259–270. doi: 10.1016/j.cell.2015.06.017

Figure 7. mTORC1 Signaling Has Opposing Effects on Cell Proliferation in Nutrient-Rich and Nutrient-Depleted Conditions.

Figure 7

(A), (B) Cell numbers of K-RasG12D MEFs (A) ±250 nM torin 1, (B) expressing Raptor or control shRNA, at day 3 of culture in medium containing 3% albumin and indicated amounts of EAAs. (C) Proliferation of pancreatic tumor cells in control and rapamycin-treated KPC mice, analyzed by immunohistochemistry against Ki-67. Scale bars = 400 μm; scale bars in blow-ups = 50 μm. (D) Quantification of Ki-67-positive tumor cells in outer and inner tumor regions as shown in (C). (E) Volume increase of pancreatic tumors in control and rapamycin-treated KPC mice, quantified by 3d high-resolution ultrasound. (F) Growth curve of Raptor KO MEFs in leucine-containing or free medium + 3% albumin. (G) Cell numbers of wild type MEFs expressing Raptor or control shRNA at day 3 of culture in medium containing 3% albumin and indicated amounts of EAAs.

Data in (A), (B), (F), (G) are represented as mean ± STDEV (n = 3). Dashed lines indicate starting cell number. Data in (D), (E) are represented as mean ± SEM (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001. See also Fig. S7.