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. Author manuscript; available in PMC: 2016 Jul 16.
Published in final edited form as: Mol Cell. 2015 Jul 2;59(2):176–187. doi: 10.1016/j.molcel.2015.05.032

Figure 5. Relevance of DSS1-RPA interaction for the recombination mediator activity of BRCA2-DSS1.

Figure 5

See also Figure S5.

(A) SDS-PAGE of purified BRCA2-DSS1 (lane 2), BRCA2-DSS18A (lane 3) and BRCA2 (lane 4). Size markers were run in lane 1.

(B) MPB-pulldown assay to test for the interaction between RPA and BRCA2, BRCA2-DSS1 or BRCA2-DSS18A.

(C) BRCA2, BRCA2-DSS1 and BRCA2-DSS18A were tested for their recombination mediator activity with RPA-coated ssDNA or SSB-coated ssDNA. The mean values (± s.d.) of data from 3 independent experiments were plotted.