. Author manuscript; available in PMC: 2016 Aug 1.

Published in final edited form as: J Bone Miner Res. 2015 May 22;30(8):1468–1480. doi: 10.1002/jbmr.2488

Table 3.

Two weeks of daily tibial loading increased cancellous, periosteal, and endosteal MAR and BFR of 10-week-old female pOC-ERαKO and LC mice. Dynamic histomorphometry, histology and immunohistochemistry data for tibia in female and male pOC-ERαKO and LC mice

		Female		Male
		LC	pOC-ERαKO	LC	pOC-ERαKO
Cn.MS	Control	0.210 ± 0.033^bc	0.231 ± 0.040^c	0.276 ± 0.046	0.250 ± 0.059
Cn.MS	Loaded	0.325 ± 0.041^†^ab	0.279 ± 0.034^†^a	0.320 ± 0.025^†	0.279 ± 0.086^†

Cn.MAR (μm/day)	Control	1.51 ± 0.14^b	1.18 ± 0.12^*^b	1.09 ± 0.13	1.01 ± 0.53
Cn.MAR (μm/day)	Loaded	1.85 ± 0.33^†^ab	2.11 ± 0.31^†^a	1.19 ± 0.18	1.10 ± 0.38

Cn.BFR (yr⁻¹)	Control	0.0550 ± 0.017	0.0502 ± 0.017	0.0331 ± 0.016	0.0266 ± 0.018
Cn.BFR (yr⁻¹)	Loaded	0.0897 ± 0.041^†	0.0396 ± 0.014^†	0.0825 ± 0.014	0.0270 ± 0.013

Es.MS	Control	0.587 ± 0.029	0.551 ± 0.186	0.357 ± 0.19	0.195 ± 0.12^*
Es.MS	Loaded	0.556 ± 0.16	0.380 ± 0.10	0.312 ± 0.19^†	0.137 ± 0.059^*^†

Es.MAR (μm/day)	Control	0.825 ± 0.19	0.710 ± 0.37	0.438 ± 0.37	0.320 ± 0.37
Es.MAR (μm/day)	Loaded	2.08 ± 0.57^†	1.47 ± 0.43^†	0.529 ± 0.72	0.143 ± 0.35

Es.BFR (yr⁻¹)	Control	0.0669 ± 0.014^b	0.0605 ± 0.038^*^b	0.0241 ± 0.017	0.0106 ± 0.015
Es.BFR (yr⁻¹)	Loaded	0.198 ± 0.076^†^a	0.105 ± 0.037^*^†^a	0.0323 ± 0.045	0.00282 ± 0.007

Es.Wo.Ar (mm²)	Control	0.00 ± 0.0	0.00 ± 0.0	0.00 ± 0.0	0.00 ± 0.0
Es.Wo.Ar (mm²)	Loaded	0.0105 ± 0.015^†	0.0223 ± 0.014^†	0.00 ± 0.0	0.00 ± 0.0

Ps.MS	Control	0.105 ± 0.060	0.0827 ± 0.032	0.0839 ± 0.045	0.211 ± 0.17
Ps.MS	Loaded	0.226 ± 0.081^†	0.260 ± 0.11^†	0.431 ± 0.23^†	0.413 ± 0.15^†

Ps.MAR (μm/day)	Control	0.00 ± 0.0	0.00 ± 0.0	0.00 ± 0.0	0.147 ± 0.36
Ps.MAR (μm/day)	Loaded	2.34 ± 0.19^†	1.70 ± 0.91^†	1.02 ± 0.95^†	0.775 ± 0.48^†

Ps.BFR (yr⁻¹)	Control	0.00 ± 0.0	0.00 ± 0.0	0.00 ± 0.0	0.00596 ± 0.015
Ps.BFR (yr⁻¹)	Loaded	0.0964 ± 0.019^†	0.0774 ± 0.040^†	0.0552 ± 0.053	0.0270 ± 0.019

Ps.Wo.Ar (mm²)	Control	0.00 ± 0.0	0.00 ± 0.0	0.00 ± 0.0	0.00 ± 0.0
Ps.Wo.Ar (mm²)	Loaded	0.140 ± 0.028^†	0.166 ± 0.049^†	0.00525 ± 0.012	0.00 ± 0.0

N.Ob/BS (mm⁻¹)	Control	2.48 ± 1.0	1.27 ± 0.23	1.68 ± 0.42	1.67 ± 0.41
N.Ob/BS (mm⁻¹)	Loaded	3.02 ± 1.0^†	2.84 ± 0.69^†	2.77 ± 0.99^†	2.57 ± 0.48^†

N.Oc/BS (mm⁻¹)	Control	2.48 ± 1.3	1.97 ± 0.98	2.90 ± 0.93	3.47 ± 0.91
N.Oc/BS (mm⁻¹)	Loaded	2.41 ± 0.87	2.35 ± 0.91	3.85 ± 1.4^†	4.38 ± 0.66^†

GP.Th (μm)	Control	107 ± 8.5	119 ± 2.7^*	106 ± 15	119 ± 10
GP.Th (μm)	Loaded	178 ± 5.0^†	198 ± 17^*^†	187 ± 15^†	180 ± 34^†

Data are mean ± SD. Cn, cancellous; Es, endosteal; Ps, periosteal; MS, mineralizing surface; MAR, mineral apposition rate; BFR, bone formation rate; N.Ob/BS and N.Oc/BS, number of matrix-secreting osteoblasts positively-stained for procollagen 1 or positively-stained osteoclasts with TRAP normalized to bone surface; GP.Th, growth plate thickness

pOC-ERαKO different from LC,

^†

Loaded tibia different from Control, p<0.05 by repeated measures ANOVA with interaction for each sex.

^a,b,c

Groups not sharing the same letter are significantly different by Tukey HSD post-hoc where a > b > c. Post-hoc performed when ANOVA interaction term was significant.