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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Acquir Immune Defic Syndr. 2015 Jul 1;69(3):278–285. doi: 10.1097/QAI.0000000000000609

Figure 1. Effect of Mab against LFA-1 on transmission of cell-associated HIV-1 across an epithelial monolayer.

Figure 1

Figure 1

Mouse anti-human CD11a (38), mouse anti-human CD18 (H52), or mouse IgG1 at concentrations of 0.02 μM or 0.004 μM were mixed with 5 × 105 HIV-1-infected PBMCs 1 hr prior to the addition of the mixture to the apical side of HT-3 cervical monolayers grown on permeable transwell supports. After 24h (A) cells in the basal compartment were counted and (B) p24 concentrations in the basal compartment were measured and percent reduction was determined compared to control IgG. All groups treated with a Mab had significantly lower number of cells and of p24 concentrations in the basal chamber compared to the IgG control group. The reduction in cell number significantly correlated (r=0.73, p=0.001) with the reduction in p24 concentration.