Table 1.
Neutralization of Env glycosylation mutants by nMAbs
| nMAbs | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Env | Region | 2F5 | 4E10 | b12 | VRC03 | VRC01 | PG9 | PG16 | 3869 |
| FE(WT) | - | 2.224 (100) | 2.986 (100) | 0.088 (100) | 2.601 (100) | 5.500 (100) | 1.415 (100) | 7.600 (100) | >25 (100) |
| N197D | V1/V2 | 0.396 (562) | 0.477 (626) | 0.005 (1760) | 0.070 (3716) | 2.355 (234) | 16.123 (9) | >25 (<30) | 5.540 (>451) |
| N301Q | V3 | 0.210 (1059) | 0.585 (510) | 0.040 (220) | 1.235 (211) | 2.330 (236) | 0.875 (162) | 14.884 (51) | ND |
| N339Q | C3 | 1.127 (197) | 1.579 (189) | 0.083 (106) | 1.799 (145) | 4.899 (112) | 1.664 (85) | 14.644 (52) | ND |
| N442Q | C4 | 0.781 (285) | 0.934 (320) | 0.045 (196) | 0.791 (329) | 4.120 (133) | 1.650 (86) | 16.111 (47) | ND |
| N448Q | C4 | 0.635 (350) | 0.694 (430) | 0.057 (154) | 2.917 (89) | 4.987 (110) | 0.878 (161) | 13.386 (57) | ND |
| N463Q | V5 | 0.763 (291) | 0.895 (334) | 0.056 (157) | 2.170 (120) | 3.450 (159) | 2.544 (56) | 16.333 (47) | ND |
| N466Q | V5 | 1.339 (166) | 1.397 (214) | 0.087 (101) | 2.139 (122) | 3.204 (172) | 3.070 (46) | 16.378 (46) | ND |
| N611Q | gp41 | 1.836 (121) | 0.704 (424) | 0.116 (76) | 3.103 (84) | 5.431 (101) | 1.797 (79) | 15.830 (48) | ND |
| N625Q | gp41 | 0.400 (556) | 0.577 (518) | 0.041 (215) | 3.329 (78) | 5.398 (102) | 1.069 (132) | 16.450 (46) | ND |
| M46 | combination | 0.435 (511) | 0.628 (475) | 0.149 (59) | 1.734 (150) | 3.683 (149) | 2.160 (66) | 16.560 (46) | ND |
| 197M.2 | combination | 0.237 (938) | 0.329 (908) | 0.008 (1100) | 0.031 (8390) | 2.685 (205) | 12.694 (11) | >25 (<30) | ND |
| 197M.3 | combination | 0.363 (613) | 0.389 (768) | 0.004 (2200) | 0.003 (86700) | 0.020 (27500) | 16.105 (9) | >25 (<30) | ND |
| 197M.4 | combination | 0.486 (458) | 0.706 (423) | 0.004 (2200) | 0.003 (86700) | 0.018 (30556) | 15.755 (9) | >25 (<30) | ND |
| 197M.5 | combination | 0.372 (598) | 0.585 (510) | 0.005 (1760) | 0.003 (86700) | 0.020 (27500) | 16.360 (9) | >25 (<30) | ND |
| 197M.6 | combination | 0.284 (783) | 0.523 (571) | 0.006 (1467) | 0.043 (6049) | 2.043 (269) | 16.230 (9) | >25 (<30) | ND |
| 197M.7 | combination | 0.253 (879) | 0.399 (748) | 0.005 (1760) | 0.023 (11309) | 1.564 (352) | 16.378 (9) | >25 (<30) | ND |
| 197M.8 | combination | 0.284 (783) | 0.463 (645) | 0.004 (2200) | 0.003 (86700) | 0.017 (32353) | 15.611 (9) | >25 (<30) | ND |
| 197M.9 | combination | 0.457 (487) | 0.700 (427) | 0.005 (1760) | 0.003 (86700) | 0.014 (39286) | 16.580 (9) | >25 (<30) | ND |
| 197M.10 | combination | 0.464 (479) | 0.886 (337) | 0.004 (2200) | 0.002 (130050) | 0.017 (32353) | 16.177 (9) | >25 (<30) | ND |
| 197M.11 | combination | 0.435 (511) | 0.536 (557) | 0.003 (2933) | 0.002 (130050) | 0.013 (42308) | 16.325 (9) | >25 (<30) | ND |
Both IC50(µg/mL) and Percentage of neutralization sensitivity relative to WT (%) were showed in each box.
Percentage of neutralization sensitivity relative to WT (%) = IC50 WT / IC50 mutant.
ND: not detected