Table 2.
Antibodies Used for Immunohistochemistry
| Antigen; product name | Host | Isot. | Target | Source |
|---|---|---|---|---|
| PAB597 | m | NA | JCV VP1 | |
| SV40 T Ag (v-300); sc-20800 | r | IgG | JCV T Ag | Santa Cruz Biotechnology, Santa Cruz, CA |
| MAP-2; HM-2; M4403 | m | IgG1 | Neurons | Sigma, St-Louis, MO |
| MAP-2; ab5622 | r | IgG | Neurons | Chemicon, Temecula, CA |
| MAP-2; LS-B290 | ch | IgY | Neurons | Lifespan Bioscience, Seattle, WA |
| MAP-2; ab5392 | ch | IgY | Neurons | Abcam, Cambridge, MA |
| NeuN (FOX3); ab104225 | r | IgG | Neurons | Abcam |
| NeuN (FOX3); ABN90P | gp | IgG | Neurons | EMD Millipore, Billerica, MA |
| GFAP; M0761; 6F2 | m | IgG1 | Astrocytes | Dako, Carpinteria, CA |
| GFAP; Z0334 | r | IgG | Astrocytes | Dako |
| GFAP; ab4674 | ch | IgY | Astrocytes | Abcam |
| GFAP; ab90601 | sh | IgG | Astrocytes | Abcam |
| GFAP; 173004 | gp | IgG | Astrocytes | Synaptic Systems, Göttingen, DE |
| CNPase; C5922; 11–5B | m | IgG1 | Myelin/Oligo | Sigma |
| CNPase; ab18527 | r | IgG | Myelin/Oligo | Abcam |
| CNPase; ab50739 | ch | IgY | Myelin/Oligo | Abcam |
| CNP; HPA023266 | r | IgG | Myelin/Oligo | Sigma |
| CD8; 1A5; VP-C325 | m | IgG1 | CD8 | Vector Laboratories, Burlingame, CA |
| CD8 (144B); ab17147 | m | IgG1 | CD8 | Abcam |
| CD3; CD3–12; OASA11552 | rat | IgG | CD3 | Aviva Systems Biology, San Diego, CA |
| CD3; CD3–12; MCA1477 | rat | IgG | CD3 | AbD Serotec, Raleigh, NC |
| CD68; M0814; KP1 | m | IgG1 | Monocytes/ Macrophages |
Dako |
| Granzyme B (11F1); VP-G812 | m | IgG2a | Granzyme B | Vector Laboratories |
| Perforin (5B10); VP-P967 | m | IgG1 | Perforin | Vector Laboratories |
| MHC class I (F-3), sc-55582 | m | IgG2a | MHC class I | Santa Cruz Biotechnology |
| MHC class I (H-300), sc-25619 | r | IgG | MHC class I | Santa Cruz Biotechnology |
| A (EP1395Y); ab52922HLA- | r | IgG | MHC class I | Abcam |
ch, chicken; CNP and CNPase, CNP, 2’,3’-cyclic-nucleotide 3’-phosphodiesterase; GFAP, glial fibrillary acidic protein; JCV, JC virus; m, mouse; MAG, myelin-associated glycoprotein; MAP-2, microtubule-associated protein 2; MOG, myelin-oligodendrocyte glycoprotein; oligo, oligodendrocytes; r, rabbit; SV40 T Ag, Simian virus 40 T antigen.