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. Author manuscript; available in PMC: 2015 Jul 19.
Published in final edited form as: J Immunol. 2015 Apr 10;194(10):4737–4749. doi: 10.4049/jimmunol.1402312

FIGURE 1.

FIGURE 1

SCARB2 is preferentially expressed in pDCs and localizes in late endosome/lysosome. (A) The expression profile of SCARB2 in human leukocytes based on cDNA data. (B) Human PBMCs, pDC-depleted PBMCs, and pDCs were isolated from healthy volunteers. The level of SCARB2 transcript was quantified by real-time PCR and normalized with GAPDH. (C) Intracellular FACS staining of SCARB2 was carried out in human PBMCs. pDCs are identified by their BDCA2 expression (circled). Matched isotype IgG was used as a control (left panel). (D) Purified pDCs were stained intracellularly with anti-SCARB2 and early (upper panel) or late (lower panel) endosome markers. The subcellular distribution of SCARB2 was shown by confocal microscopy. Nucleus was stained by DAPI (blue). Results are representative of at least three independent experiments. Scale bars, 5 μm.