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. Author manuscript; available in PMC: 2015 Jul 19.
Published in final edited form as: J Immunol. 2015 Apr 10;194(10):4737–4749. doi: 10.4049/jimmunol.1402312

FIGURE 3.

FIGURE 3

SCARB2 regulates TLR9-dependent IFN-I production. (A and B) GEN2.2 cells were transduced with lentivirus carrying either scrambled shRNA (sh-c) or shRNA targeting SCARB2 (sh-1/2), and stable cell lines were generated. The knockdown efficiency was confirmed by real-time PCR (A) and Western blotting (B). GAPDH or β-actin was used as a housekeeping control, respectively. SCARB2 knockdown (sh-1/2) and control cells (sh-c) were stimulated with CpG A (C) or CpG B (D) for 20 h. Levels of IFN-α (left panel) and IL-6 (right panel) in the culture supernatants were measured by ELISA. (E) SCARB2 knockdown (sh-1/2) and control cells (sh-c) were stimulated with CpG B for the indicated durations. Cells were collected, and the levels of IFN-α transcript were detected by real-time PCR normalized with GAPDH as a housekeeping control (left panel). Levels of IFN-α in the culture supernatants were measured by ELISA (right panel). Results are representative of at least three independent experiments. The data are presented as the mean ± SEM of duplicates (ns indicates not significant).