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. Author manuscript; available in PMC: 2015 Jul 19.
Published in final edited form as: J Immunol. 2015 Apr 10;194(10):4737–4749. doi: 10.4049/jimmunol.1402312

FIGURE 6.

FIGURE 6

SCARB2 is not required for CpG B endocytosis and trafficking. (A) SCARB2 knockdown (sh-1) and control cells (sh-c) were stimulated with biotin-conjugated CpG B. Endocytosis of CpG B was detected by intracellular FACS staining. Left panel was a representative histogram of CpG B internalization in control (sh-c, black line) and SCARB2 knockdown cells (sh-1, red line) at 1 h after stimulation. Right panel was the summary data of 1, 2, and 4 h post-CpG B stimulation. (B) SCARB2 knockdown (sh-1) and control cells (sh-c) were mixed together with equal amounts and stimulated with biotin-conjugated CpG B for 1 h. Intracellular SCARB2 and CpG B were stained with anti-SCARB2 and Cy5 streptavidin, respectively. White arrows indicate to sh-c control cells, whereas purple arrows indicate to sh-1 SCARB2 knockdown cells. (C) SCARB2 knockdown (sh-1) and control cells (sh-c) were stimulated with biotin-conjugated CpG B for 5 min, 1 h, and 3 h. CpG B trafficking was confirmed by intracellular staining together with early or late endosome marker. Nucleus was stained by DAPI (blue). Results are representative of at least three independent experiments. Scale bars, 5 μm.