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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Immunol. 2015 Jun 15;195(3):924–933. doi: 10.4049/jimmunol.1500070

FIGURE 8. Increased IL-10 induced by DB06-1.

FIGURE 8

(A, B) IL-10 production as measured by intracellular staining of splenic iNKT cells from C57BL/6 mice. iNKT cells were gated as live cells that were B220−, CD8α−, CD3ε+ and CD1d-tet+. Mice were injected with 4 μg of the indicated Ags, DB06-1 or αGalCer one month prior. Cells were re-stimulated with PMA and ionomycin in vitro and intracellular IL-10 produced by CD4+ and DN iNKT cells is shown. (A) Representative flow cytometry plots showing IL-10 production and (B) summary graph. Data are representative of two independent experiments. Error bars represent ± SEM of at least three mice per condition.