Skip to main content
. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Immunol. 2015 Jun 24;195(3):944–952. doi: 10.4049/jimmunol.1500443

Figure 1.

Figure 1

Aged Treg are enriched for Nrp-1+ and Helios+ cells, and maintain a similar TCR Vβ usage. Splenocytes from young (3 months), middle-aged (12 months), and old (18 months) wild-type mice were stained for CD4, FoxP3, CD44, CD62L, Nrp-1, and Helios and analyzed by flow cytometry (n=4 mice/group). A, Representative dot plots show the expression of Nrp-1 and Helios in total CD4+ FoxP3+ cells. Numbers are the frequency of CD4+ FoxP3+ cells. Scatter plots show the frequency and total number of CD4+ FoxP3+ Treg that are Nrp-1+ Helios+, Nrp-1+ Helios−, Nrp-l− Helios+, or Nrp-1− Helios−. B, Splenocytes from young (3 months, n=4), and old (22 months, n=4) mice were stained for CD4, FoxP3, and Vβ2-17a and analyzed by flow cytometry. Data shows the average frequency of CD4+ FoxP3+ cells that are Vβx+ (±SE). The p values represent the difference between young and middle-aged or old mice (**p ≤ 0.01, Student’s t test). Data is representative of at least three independent experiments.