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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Immunol. 2015 Jun 24;195(3):944–952. doi: 10.4049/jimmunol.1500443

Figure 2. Aged Treg display the transcriptional signature of “effector” Treg.

Figure 2

Splenocytes from young (3–5months) and old (>18months) FoxP3-IRES-GFP reporter mice were sorted for CD25hi Treg (CD4+ FoxP3GFP+ CD25hi), CD25lo Treg (CD4+ FoxP3GFP+ CD25lo), memory CD4+ T cells (CD4+ FoxP3GFP− CD44hi CD62Llo), and naïve CD4+ T cells (CD4+ FoxP3GFP− CD44lo CD62Lhi), and the isolated RNA was sent for NextGeneration RNA sequencing. A, Heatmaps show the relative gene expression. B, Principle component analysis is shown.