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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Immunol. 2015 Jun 24;195(3):1242–1250. doi: 10.4049/jimmunol.1500243

Figure 4.

Figure 4

KIR3DL1*009 binds poorly to a panel of HLA-Bw4 antigens. A, Soluble KIR-Fc (4.0 µg/ml) binding to a panel of 12 HLA Bw4 80I antigens, 6 HLA Bw4 80T and 31 HLA Bw6 antigens was assessed using a bead-based multiplex platform. Each data point represents binding levels to an individual HLA molecule. The binding was performed in the presence or absence of DX9 as indicated. B, Soluble wild-type KIR3DL1*001 and KIR3DL1*009 binding to a multiplex of HLA antigens was compared to the binding of the mutant, KIR3DL1*001 S58G, V92M. The data are presented as a normalized ratio accounting for the total amount of HLA conjugated to each bead as described in the methods section. These experiments were performed in triplicate and repeated in three independent experiments. The data were analyzed by one-way ANOVA followed by a Tukey’s multiple comparisons test (vs 3DL1*001-Fc, * P < 0.05, *** P < 0.001).