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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Immunol. 2015 Jun 24;195(3):1242–1250. doi: 10.4049/jimmunol.1500243

Figure 6.

Figure 6

KIR3DL1*009 positive NK cells exhibit greater response to HLA-Bw4 positive target cells. PBMCs from a healthy donor heterozygous for KIR3DL1*001 and KIR3DL*009 as well as PBMCs from a KIR3DL1*005 positive donor were challenged with HLA negative 721.221 cells or 721.221 cells expressing HLA-B*44:03 in the presence or absence of the blocking DX9 antibody. Activation was determined independently for each KIR3DL1 positive population using Z27. The responses for each KIR3DL1 population were normalized to activation observed for each population after co-incubation with the parental 721.221 cells. These experiments were performed in triplicate and repeated in three independent experiments. The results were analyzed using a one-way ANOVA followed by a Tukey’s multiple comparisons test (vs 3DL1*009, ** P < 0.01, *** P < 0.001; ns, not significant).