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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Immunol. 2015 Jun 24;195(3):1202–1217. doi: 10.4049/jimmunol.1403013

Figure 5. Cytokine production by BMDMs and BMDCs is independent of SOCE.

Figure 5

(a) WT and Stim1fl/flStim2fl/fl Vav-Cre BMDMs were stimulated with 1 μg/ml LPS, 50 μg/ml curdlan, or 5 MOI BCG for 16 h or left untreated (unstim.). Levels of TNFα, IL-6, IL-10, IL-12p70, IL-2, and IL-12/23p40 in the cell supernatant were measured by ELISA. Bar graphs represent the mean ± SEM of 3 mice per group. (b) WT and Stim1fl/flStim2fl/fl Vav-Cre BMDCs were stimulated with 1 μg/ml LPS, 100 nM CpG, 25 μg/ml zymosan, 50 μg/ml curdlan, 1 μg/ml imiquimod for 16 h or left untreated (unstim.). Levels of TNFα, IL-6, IL-10, IL-12p70, IL-2, and IL-23p19 in cell supernatants were analyzed by ELISA. Bar graphs represent the mean ± SEM of 8 mice per group.