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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Immunol. 2015 Jun 26;195(3):1112–1120. doi: 10.4049/jimmunol.1500084

Figure 2. Similar patterns of CD8+ T cell memory generation in response to Listeria infection from both NKT and conventional T cell compartments.

Figure 2

Mice were infected with 104 CFU of LM-Ova on day 0 and re-challenged with 2x105 CFU on day 42, as indicated by arrows in (C). (A) Plots show the distribution of CD62L versus CD127 among gated Ova-tetramer+ CD8+ NKT or CD8+ T cells 7 and 28 days post-boost. (B) Graphs summarize the frequency of effector, central memory, and effector memory cells among Ova-tetramer+ CD8+ NKT or CD8+ T cells on day 7 post-prime and days 7 and 28 post-boost. (C) At the indicated timepoints, cells from spleen and liver were re-stimulated ex-vivo with Ova or LemA peptides. Graph show the kinetics of the frequency of IFNγ-expressing cells among CD8+ NKT versus CD8+ T cells. (D) At the indicated timepoints, granzyme B expression was measured in CD8+ NKT or CD8+ conventional T cells. Data are representative from one of two independent experiments, n = 3 for each, mean ± s.e.m (A–D). *p < 0.05, **p < 0.01 between NKT and conventional CD8+ T cells.