Fig. 4.

Loss of anillin induces disorganization of the perijunctional actomyosin belt. DU145 cells transfected with either control or anillin-specific siRNAs were subjected to dual labeling for either a filamentous (F) actin (green) and β-catenin (red) or b NM IIB (green) and E-cadherin (red) on day 4 post-transfection. Arrows indicate a well-formed perijunctional actomyosin belt in control cells. Arrowheads highlight disorganized perijunctional actin filaments and basal NM IIB-enriched stress fibers in anillin-depleted cells. Scale bar 20 µm