Fig. 8.

Activation of JNK mediates junctional disassembly in anillin-depleted epithelial cells. a Expression and phosphorylation of three major MAPKs was determined in control and anillin-depleted DU145 cells via immunoblotting. b, c Control and anillin-depleted DU145 cells were treated for 24 h with either vehicle or JNK inhibitor, SP600125 (25 µM). The effect of JNK inhibition on AJ and TJ integrity was determined by immunolabeling of E-cadherin and ZO-1. JNK activation and expression of γ-adducin was examined by immunoblotting. Arrows indicate AJ/TJ disassembly in vehicle-treated anillin-depleted cells. Arrowheads highlight restoration of junctional integrity of anillin-depleted cells by JNK inhibition. Scale bar 20 µm