Skip to main content
. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Immunol. 2015 Jun 24;195(3):853–864. doi: 10.4049/jimmunol.1403114

Figure 4. IgM and IgG anti-insulin antibodies are produced in VH125SD B6 mice following TI but not TD immunization.

Figure 4

VH125SD and WT B6 mice were immunized with either bovine insulin in 1X PBS emulsified in CFA (insulin/CFA) s.c. base of the tail, or Brucella abortus ring test Ag (BRT) alone, or human insulin conjugated to BRT (insulin-BRT) i.p. in 1X PBS. (A) Insulin-specific IgMa, IgG1a, and IgG2aa (VH125SD) or IgMb, IgG1b, and IgG2cb (WT) in sera was measured by ELISA before (open) and 2–3 wks following TD immunization with insulin/CFA (closed). (B) Insulin-specific IgM, IgG1, and IgG2a/c in sera was measured by ELISA 5–7 d following immunization with BRT alone (open) or insulin-BRT (closed). For insulin/CFA, n = 12 for both VH125SD and WT. For insulin-BRT, n = 12 VH125SD and n = 10 WT. For BRT alone, n = 6 mice per group. Data represent at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, two-tailed t test.