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. Author manuscript; available in PMC: 2016 Aug 7.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Jun 5;463(4):811–817. doi: 10.1016/j.bbrc.2015.04.153

Figure 4. Colocalization of tight junction proteins.

Figure 4

Representative fluorescent images of isolated brain microvessels from sedentary and exercised mice at (A) 48 hours (B) 3 weeks post tumor cell infusion. Occludin (green), and ZO-1 (red) immunreactivity was evaluated using a Nikon Eclipse Ti-U fluorescence microscope and NES Elements software at 40X magnification. Merged images show occludin and ZO-1 overlap as well as nuclei (stained with DAPI, blue).