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. 2015 Jun 22;5:542–549. doi: 10.1016/j.fob.2015.06.007

Fig. 2.

Fig. 2

YAP/TAZ-dependent breast cancer cell lines are sensitive to dasatinib, fluvastatin, and pazopanib. (A) Sensitivity of breast cancer cell lines to (i) dasatinib, (ii) fluvastatin, and (iii) pazopanib. Cells were treated with drugs for 4 days. Viability was measured by MTT assay. Data represents mean and standard deviation from triplicate. Data is representative of at least three independent experiments. HEK293 (black line) was used as normal cell control. (B) Activation states of YAP/TAZ-dependent transcription in breast cancer cell lines. Cells were transfected with 8xGTIIC-luciferase and pRL-CMV vector. Promoter activity was measured 2 days after transfection. Data represents mean and standard deviation from triplicate. Data is representative of at least two independent experiments. (C) Expression of YAP and TAZ in breast cancer cell lines. Asterisks show phosphorylated YAP and TAZ. (D) Dependence of cell growth on YAP and TAZ. Cells were transfected with siRNA for YAP and TAZ and were diluted in 6 well plates. Colony formation was measured by crystal violet. Data is representative of at least two independent experiments.