Fig. 1.

In situ hybridization patterns for Bm1_20330 in adult B. malayi adult worms. The sense RNA probe (negative control) did not label tissues in female (A) or male worms (F). In contrast, the antisense probe produced strong signals in female (B–E) and male worms (G–I). Oocytes in ovary (B), morulae stage embryos (C) and the uterine wall (arrow in E) adjacent to pretzel or stretched microfilariae (Mf) were intensely labeled and stretched Mf was partially labeled (E). The antisense probe also strongly labeled spermatogonia in the male testis (G, H) and the wall of the Vas deferens (arrow) (I), the lateral cord was weakly labeled (G, H). Weak to moderate labeling was also observed in both male and female body muscle. Abbreviations: Ov, ovary; I, intestine; U, uterus; M, muscle; Lc, lateral cord, Vd, Vas deferens; T, testis. Scale bar 10 μm in panel A–E and 5 μm in panel F–I.