Fig. 2.

In situ hybridization patterns for Bm1_38195 in adult B. malayi adult worms. The sense RNA probe (negative control) did not label tissues in female (A) or male worms (F). In contrast, the antisense probe produced moderate to strong signals in female (B–E) and male worms (G–I). Oocytes in ovary (B), morulae stage embryos (C), and the uterine wall (E, arrow) adjacent to pretzel or stretched Mf were intensely labeled; and pretzel stage and stretched Mf were partially labeled (D, E). The antisense probe also strongly labeled spermatogonia in the male testis (G) and the wall of the Vas deferens (arrow) (I), the lateral cord was weakly labeled in female (B, C) and male (H, I), respectively. Weak to moderate labeling was also observed in both female and male body muscle. Abbreviations: Ov, ovary; I, intestine; U, uterus; M, muscle; Lc, lateral cord, Vd, Vas deferens; T, testis. Scale bar 10 μm in panel A–E and 5 μm in panel F–I.