Fig. 4.

In situ hybridization patterns for Bm1_48815 in adult B. malayi adult worms. The sense RNA probe (negative control) did not label tissues in female (A) or male worms (F). In contrast, the antisense probe produced strong signals in female (B–E) and male worms (G–I). Oocytes in ovary (B), morulae stage embryos (C) and the uterine wall adjacent to pretzel or stretched Mf (E, arrow) were intensely labeled; pretzel stage (D) and stretched Mf (E) were partially labeled. The antisense probe also strongly labeled spermatogonia in the male testis (G, H) and the wall of the Vas deferens (arrow) (I); the lateral cord was strongly labeled in male (G, H). Weak to moderate labeling was also observed in male body muscle. Abbreviations: Ov, ovary; I, intestine; U, uterus; M, muscle; Lc, lateral cord, Vd, Vas deferens; T, testis. Scale bar 10 μm in panel A–E and 5 μm in panel F–I.