Localization of the predicted peptide from Bm1_48815 in adult B. malayi adult by immunohistochemical labeling. Negative control did not label any tissue except areas where the internal enzymes are high such as intestine in female (A) or male (F). Polyclonal antibodies against a predicted peptide from Bm_48815 labeled developing embryos, lateral cord, the wall of uteri with stretched Mf (arrow) in female (B–D); the antibody also labeled spermatogonia (G) and the body muscle and lateral cord (G–I), and the wall of the Vas deference with mature sperms (arrow, I). Abbreviations: Ov, ovary; I, intestine; U, uterus; M, muscle; Lc, lateral cord, Vd, Vas deferens; T, testis. Scale bar 10 μm in panel A–E and 5 μm in panel F–I.