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Trypticase-phytone-yeast extract liquid culture medium composition
| Components | Weight (g/L) |
| Casein hydrolysate | 1.0 |
| Soya bean peptone | 5.0 |
| Yeast powder | 2.0 |
| Glucose | 5.0 |
| L-Cysteine | 0.5 |
| K2HPO4 | 2.0 |
| MgCl2 | 0.5 |
| ZnSO4 | 0.25 |
| CaCl2 | 0.15 |
| FeCl3 | 0.000001 |
| Tween-80 | 1.0 |
| pH | 6.5 ± 0.1 |
