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. 2014 Dec 22;21(13-14):1973–1981. doi: 10.1089/ten.tea.2014.0381

FIG. 4.

FIG. 4.

Accumulation of SZP stimulated by TGF-β1 after enzymatic removal and regeneration of endogenous GAG. Monolayer superficial zone articular chondrocyte cultures were treated with HEP-I or C-ABC for the enzymatic removal of endogenous HS or CS, respectively. After enzymatic treatment, cultures were treated with either 0.3 or 1 ng/mL of TGF-β1 immediately or incubated for 72 h to allow regeneration of endogenous GAGs before treatment with either 0.3 or 1 ng/mL of TGF-β1. Cultures were treated for 4 days after initial TGF-β1 treatment. (A) SZP accumulation significantly increased with TGF-β1 (0.3 ng/mL) in the absence of endogenous GAGs. (B) SZP accumulation significantly increased with TGF-β1 (1 ng/mL) in the absence of endogenous CS. The replenishment of endogenous HS enhanced SZP accumulation by 1.3-fold. Results presented as SZP protein fold increase compared with untreated controls. Values presented as mean±standard error. *p<0.05 compared with untreated control.