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. 2014 Dec 22;21(13-14):1973–1981. doi: 10.1089/ten.tea.2014.0381

FIG. 5.

FIG. 5.

Accumulation of SZP stimulated by TGF-β1 in the presence of exogenous GAGs after enzymatic removal of endogenous GAGs. Monolayer superficial zone articular chondrocyte cultures were treated with HEP-I or C-ABC for the enzymatic removal of endogenous HS or CS, respectively. After enzymatic treatment, cultures were treated for 4 days with either 0.3 or 1 ng/mL of TGF-β1 in the presence of 30 μg/mL of GAGs. (A) TGF-β1 (0.3 ng/mL) in the presence of replenished exogenous heparin and CS significantly increased SZP accumulation in the culture media. (B) TGF-β1 (1 ng/mL) in the presence of replenished exogenous CS significantly increased SZP accumulation in the culture media. Results presented as SZP protein fold increase compared with untreated controls. Values presented as mean±standard error. *p<0.05 compared with untreated control.