FIG. 5.

GFAP⁺ astrocytes and Iba1⁺ macrophage/microglia at 4 weeks postinjury. GFAP expression extends into the distal processes of astrocytes in animals with transection only (A), but is restricted to the cell bodies and proximal processes of astrocytes in animals with implanted polymer scaffolds (B) (Scale bars: 50 μm). Iba1 (red) and MBP (green) staining within the lesions of animals with implanted polymer scaffolds containing Matrigel only (C), with implanted polymer scaffolds containing Schwann cells (D), and with transection only (E) (Scale bars: 50 μm). (F): Quantification of the proportion of the area within one mm of the stromal scar that is GFAP⁺ in animals with transection only and with implanted polymer scaffolds. (G): Amoeboid Iba1⁺ cell counts within the lesions of animals with implanted polymer scaffolds containing Matrigel only, with implanted polymer scaffolds containing Schwann cells, and with transection only. GFAP, glial fibrillary acidic protein. Color images available online at www.liebertpub.com/tea