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. 2015 Jul 20;5:10654. doi: 10.1038/srep10654

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Sequencing results of randomized position S72 (WT codon: AGT) obtained from single colonies formed on agar plates. Vertical axes denote counts (how many codons of each type were found in the sequencing). Black columns denote desalted primers, and grey ones denote HPLC primers. Libraries: (a) 1-2; (b) 3-4; (c) 5-6; (d) 7-8; (e) 9-10; and (f) 11-12.