Figure 3.

Histone 3 acetylation and netrin-1 expression in HUVECs. Western blotting (A, B) and immunofluorescence (C) studies showing degree of histone acetylation in HUVECs in response to different treatments as specified. In the Western blots, densitometric analysis of acetylated H3 (acH3) was normalized to total H3. In immunofluorescence experiments, the corrected nuclear cell fluorescence (CNCF) was calculated (C). Panels (D)–(H) show the effect of aspirin (ASA) on HAT and HDAC activities in HUVECs. HAT (D) and HDAC (E) activities, and HDAC/HAT ratio (F), were measured in nuclear extracts isolated from HUVECs following treatments as specified. Nuclear extracts were isolated from HUVECs not stimulated with TNF-α, and HAT (G) and HDAC (H) activities were tested in the presence of either ASA or salicylic acid. n = 3. ASA, 0.5 mM; SC-560, 30 nM; SA: salicylic acid, 0.5 mM; Indom: indomethacin, 100 μM; TSA, 400 nM; TNF-α, 10 ng·mL⁻¹; Bay 11-7085 (BAY), 5 μM. *P < 0.05 versus CTR (control HUVECs not treated with TNF-α).