Skip to main content
. 2015 Jun 4;172(14):3565–3578. doi: 10.1111/bph.13146

Figure 1.

Figure 1

Cytotoxic effects of EGCG and its derivatives in K562 and KBM5 CML cells. (A) Chemical structures of the EGCG derivatives, EGCG (MW = 458.4), EGCG-MO (MW = 584.6), EGCG-ML (MW = 640.7) and EGCG-MP (MW = 696.9). (B) Stability of EGCG and its derivatives in culture medium was determined by HPLC. (C and D) A suspension of K562 cells at 2.0 × 105 cells·mL−1 in RPMI1640 medium was centrifuged. Then 1 mL of EGCG (40 or 200 μM) or EGCG-MP (40 μM) solution were added to the cells and incubated at 37°C with 5% CO2 for 30 min and individually dispensed to two filter-tip syringes [using 0.45 μm HLS (HTTP Live Streaming)-DISC 13 filter] and finally lysed with 1% lysis buffer for HPLC analysis. (E) K562 cells and (F) KBM5 cells were treated with 0, 5, 10, 20 and 40 μM of EGCG or EGCG derivatives for 24 h. Cell viability was measured by MTT assay. Data are presented as means ± SD. *P < 0.05; **P < 0.01, significantly different from untreated control.