Figure 3.

SAR7334 potently inhibits TRPC6 whole-cell currents. TRPC6 currents were elicited in TRPC6-HEK-FITR cells by application of 50 μM OAG. (A) Current traces measured during voltage ramps before and after application of the TRPC6 channel activator OAG and after consecutive additions of SAR7334. (B) Time course and dose dependence of SAR7334-induced TRPC6 inhibition. Whole-cell currents were measured at −70 mV during voltage ramps and plotted versus time. For better comparison of responses, current amplitudes were normalized to the maximal current after addition of OAG and maximal currents were superimposed. The arrow indicates application of SAR7334. (C) TRPC6 currents were measured as in (B) and inhibition was determined in steady state after application of the respective concentration of SAR7334. The dose–response relationship was fitted to a logistic function. Data are given as means ± SEM (n = 3–12).