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Heterologous expression and purification of BbBqrA. (A) Sixteen-percent SDS-polyacrylamide gel of recombinant BbBqrA: lane 1, molecular weight standards; lane 2, E. coli BbBqrA expression crude extract; lane 3, purified recombinant BbBqrA (3 µg). (B) Relative benzoquinone reductase activity at different pH values using 1,4-benzoquinone as the substrate in the presence of NADPH.
