Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PSGL-1, CD43, and CD44 require sialic acids to associate with lipid rafts. WT neutrophils were incubated with buffer or neuraminidase (sialidase). (A) The cells were lysed, fractionated on OptiPrep gradients, and analyzed by Western blotting with antibodies to the indicated protein as in Fig. 1. (B) CTxB-bound rafts and antibodies to the indicated protein were visualized by confocal microscopy as in Fig. 1. Results are representative of at least three experiments. (Scale bar, 5 μm.)
