Fig. 4.

PSGL-1 requires core 1-derived O-glycans to trigger SFK-dependent generation of microparticles. (A) Fluorescent WT or C1galt1^−/− neutrophils were incubated with vehicle control or with the Ca²⁺ ionophore A23187. The number of microparticles generated was measured by flow cytometry. (B) Fluorescent neutrophils of the indicated genotype were incubated with F(ab′)₂ fragments of isotype control, anti-CD45, or anti–PSGL-1 mAb. The number of microparticles generated was measured by flow cytometry. The data represent the mean ± SEM of five experiments. *P < 0.01.