Fig. S2.

The cholesterol probe filipin binds similarly to WT and C1galt1^−/− neutrophils. (A) WT or C1galt1^−/− neutrophils or (B) WT neutrophils pretreated with the cholesterol chelator methyl-β-cyclodextrin (MβCD) or its inactive analog α-cyclodextrin (αCD) were incubated with DMSO vehicle or filipin. Flow cytometry was used to measure filipin binding by UV light emission. Results are representative of three experiments.