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. 2015 Jun 29;112(28):8780–8785. doi: 10.1073/pnas.1502522112

Fig. 4.

Fig. 4.

Expression analysis of the two ZmWAK-RLK1 splice variants. (A) cDNA structure of the two splice variants ZmWAK-RLK1.1 and ZmWAK-RLK1.2. Exons are represented as rectangular bars, and the retained intron two in ZmWAK-RLK1.2 as a black line. Predicted protein domains are indicated in color. The red asterisk indicates the premature stop codon. (B and C) Relative ZmWAK-RLK1 expression levels. (B) ZmWAK-RLK1.1 in resistant lines (R lines; mix of line RP1Htn1 and three resistant recombinants) and susceptible lines (S lines; mix of RP1 and two susceptible recombinants) of 20-d-old seedlings and (C) ZmWAK-RLK1.1 (Left) and ZmWAK-RLK1.2 (Right) in resistant and susceptible mapping parents of 35-d-old seedlings, either noninfected or after E. turcicum infection. Mean (B) and untransformed mean (C) values are presented, and the 95% confidence intervals (back-transformed) are plotted. On top of the bars, different letters denote a significant difference in expression level (Tukey’s honestly significant difference test, α = 0.050).