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. 2015 Jun 29;112(28):8650–8655. doi: 10.1073/pnas.1500886112

Fig. 3.

Fig. 3.

Hypermethylation of miR-148a is involved in resistance to TRAIL-induced apoptosis. (A) qRT-PCR showing miR-148a expression in A549 cells after treatment with 5-aza-dC (1 μM and 5 μM) for 72 h. (B) Caspase-3/7 assay after treatment of A549 cells with 5-aza-dC followed by TRAIL (100 ng/mL). (C) Western blot showing PARP-1 and caspase-8 activation after 3-h TRAIL treatment (100 ng/mL) in H1299 cells treated with 5-aza-dC for 72 h. After treatment with 5-aza-dC, NSCLC cells showed increased sensitivity to TRAIL-induced cell death as indicated by increased caspase-3/7 activity and PARP-1 and caspase-8 cleavage. Data are presented as ± SD; *P < 0.05 and **P < 0.01 by Student’s t test.