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. 2015 Jun 29;112(28):8650–8655. doi: 10.1073/pnas.1500886112

Fig. S2.

Fig. S2.

miR-148a induces TRAIL sensitivity by targeting MMP15. (A) Proliferation assay in Calu-1 cells transfected with a scrambled miR or miR-148a for 48 h followed by 24 h of TRAIL treatment (400 ng/mL). Cells expressing higher levels of miR-148a showed reduced cell viability after TRAIL treatment. (B) Caspase-3/7 activity assay in Calu-1 cells transfected with control miR or miR-148a after TRAIL treatment (400 ng/mL). (C) Caspase-8 activity assay in H292 TRAIL-sensitive cells transfected with empty vector or MMP15 plasmid followed by TRAIL treatment (25 or 50 ng/mL) for 3 h. Cells expressing MMP15 showed reduced caspase-8 activation after TRAIL treatment. Proliferation assay (D) and caspase-3/7 (E) activity assay in H460 cells transfected either with an empty vector or ROCK1 plasmid. ROCK1 overexpression was not able to protect H460 cells from TRAIL-induced apoptosis. Data are presented as ± SD; *P < 0.05 and **P < 0.01 by Student’s t test. ns, not significant.