Fig. 5.

Lysosome accumulations at amyloid plaques are predominantly axonal in origin. (A) Confocal images of cortex from 3-mo-old 5xFAD mice stained for CD68 (microglial lysosome marker), Aβ (amyloid), and PGRN (lysosomes). (B) Quantification of the percentage of small Aβ deposits that have either CD68 and/or PGRN associated with them [mean ± SD, n = 3 (3-mo-old) 5xFAD mice per experiment, >40 plaques scored per mouse]. ***P < 0.001. (C) GFAP and LAMP1 staining in 5xFAD cerebral cortex. (D) 5xFAD cerebral cortex mice stained for MAP2B (dendritic marker) and LAMP1. (E) Neurofilament and LAMP1 staining in 5xFAD cerebral cortex. (Scale bars: 10 μm.) See also Fig. S3.