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. 2015 Jun 29;112(28):8632–8637. doi: 10.1073/pnas.1510929112

Fig. 1.

Fig. 1.

FBXO31 is required for decreased MDM2 and increased p53 levels following DNA damage. (A and B) Immunoblot monitoring MDM2 [using a monoclonal (AB1) or polyclonal (N20) antibody], p53, FBXO31, phosphorylated ATM [p-ATM(1981)], and total ATM (t-ATM) in MCF7 cells expressing NS or FBXO31 shRNA and treated in the presence (45 or 90 min) or absence (0 min) of camptothecin (A) or γ-irradiation (IR) (B). α-tubulin (TUBA) was monitored as loading control. (C) Immunoblot monitoring Flag-MDM2, p53, and FBXO31 in MCF7 cells expressing Flag-MDM2 and NS or FBXO31 shRNA and treated in the presence or absence of camptothecin. GFP, expressed from a cotransfected plasmid, was used as a transfection and loading control. (D) Mitotic index analysis of HCT116 cells expressing NS or FBXO31 shRNA. Error bars indicate SD. *P ≤ 0.05, **P ≤ 0.01.