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. 2015 Jun 29;112(28):8656–8660. doi: 10.1073/pnas.1506351112

Fig. S4.

Fig. S4.

Centromere defects in gip1gip2. (A) 2C and 4C sorted nuclei from roots and leaves of gip1gip2 and WT plantlets. Immunolabeling was performed using anti-CENH3 antibodies. (B) Live cell imaging showing mitotic defects in gip1gip2 overexpressing CENH3. Arrows indicate mis-segregated chromatids. (C and D) Immunostaining of gip1gip2 mitotic cells using anti-CENH3 antibodies. Arrows indicate lagging chromosomes and one micronucleus is indicated (arrowhead). (E) Quantification of the number of chromosomes per mitotic cell (n = 50). (Scale bars, 2 µm.)