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. 2015 Jul 20;5:12300. doi: 10.1038/srep12300

Figure 7. Inhibition of polyubiquitination of TAK1 at Lys562 residue leads to a decline in cytokine production.

Figure 7

A and B, TAK1 is required for TLR4-mediated induction of TNF and IL-6 mRNA. TAK1-silenced RAW264.7 cells were reconstituted with Flag-tagged TAK1 wild-type (WT), T(184,187)A, K562R or K(562,563)R mutant. After treatment with LPS for indicated times, the cells were collected and the total RNA was extracted, reverse transcribed, and analyzed for TNF and IL-6 mRNA with Q-PCR. C and D, TAK1 is required for TLR4-mediated TNF and IL-6 production. TAK1-silencing RAW264.7 cells were reconstituted with Flag-tagged TAK1 wild-type (WT), T(184,187)A, K562R or K(562,563)R mutant. After incubating with LPS for 24 h, the medium was collected and analyzed for TNF and IL-6 level with ELISA. Results are averages ± SD of three separate experiments (*, significant difference).